Part:BBa_K4275032:Design
PfuFerritin-Nb3
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 547
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The amino acid sequence of Pfuferritin is derived from the genomic sequence of Pyrococcus furiosus whilst its DNA sequence is codon-optimized based on the codon-usage table of E.coli Strain K12.MG1655.
2. An alpha-helice is omitted at the C temrinus of the original Pfuferritin, to accomodate the newly inserted Nb3 domain.
3. The ferritin domain and the Nb3 domain are interspaced by a flexible GS linker (GGGSGGGGSGGG) to avoid the disruption of the oligomerization of ferritin subunits into a 24-mer.
Source
Pyrococcus furiosus
Lama glama (Nb3)
References
1. Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217